Balfour states that she is returning Carl Hagenbeck's letter to Ewart. She states she has also written to Lord Stanley in connection with Ewart's hybrids in an attempt to persuade him to purchase them. She also enquires whether, during his experiments, he has noticed if offspring from two different species of horse, with strong markings, hark back to a more ancestral type. She enquires whether the mustang is a descendant of the Spanish horse.

Balfour writes regarding the possible publication of a German pamphlet by Professor Von Nathusius in the Veterinarian. She now believes she can have it translated by Sir Walter Rothschild at Tring in Hertfordshire. She goes on to discuss the markings of a particular breed of zebra from Zululand, and whether its unusual pattern has any bearing on the history of markings in general.

Balfour expresses her hope that the Highland and Agricultural Society gives him a grant to continue his experiments and thanks Ewart for accepting her financial assistance. She goes on to discuss the 'infection' of telegony and the positioning of animal markings and gradations in colour on zebras and horses.