Balfour writes that she has spoken to her brother about obtaining funding from the Carnegie Trust for a research farm. She asks Ewart to send her brother a letter stating what the Research Committee wishes, and he will attempt to forward this to Lord Elgin, who may be able to help. She offers to make enquiries about the whereabouts of the ponies 'Brenda' and 'Black Agnes'.

Balfour writes that her brother has been unsuccessful in attempting to gain Lord Elgin's help for funding for a research farm. She enquires whether anyone has tried procuring immunity for horses from the Tsetse fly by permitting infection in zebras.

Balfour writes regarding the possible publication of a German pamphlet by Professor Von Nathusius in the Veterinarian. She now believes she can have it translated by Sir Walter Rothschild at Tring in Hertfordshire. She goes on to discuss the markings of a particular breed of zebra from Zululand, and whether its unusual pattern has any bearing on the history of markings in general.

Balfour refers to photographs of a zebra skin she sent to Sir George Grey, who is presently staying in a British Central area of Africa, South of the Congo. She believes the skin to be from a Burchell's zebra. She also refers to a Tibetan pony belonging to Major L.A Waddell.

Balfour writes regarding Ewart's wish to obtain a particular type of horse from Norway. Lord Walter Rothschild has suggested that he might contact Ethelbert Lort-Phillips who is interested in scientific natural history and is often in Norway.

Balfour writes that she is enclosing some pages of a pamphlet relating to the hybridisation of plants and the connections with animal cross-breeding (pamphlet not present). She also mentions hearing that 'Romulus (Ewart's first zebra hybrid foal) has been taken away from you under rather trying circumstances.'

Balfour encloses a letter from Monk Bretton expressing hope that the lives of the zebras in South Africa may be spared. Balfour also thanks Ewart for sending her a copy of the Report of the Research Committee.

Balfour states she is returning Carl Hagenbeck's letter to Ewart. She states she has also written to Lord Stanley in connection with Ewart's hybrids.