Balfour writes that she is enclosing some pages of a pamphlet relating to the hybridisation of plants and the connections with animal cross-breeding (pamphlet not present). She also mentions hearing that 'Romulus (Ewart's first zebra hybrid foal) has been taken away from you under rather trying circumstances.'

White expresses interest in Ewart's article on experiments of crossing animals of different families. He discusses other cases of animal hybridisation he knows of, and says he is enclosing a clipping relating to Charles Goodnight's experiments with crossing buffalos and cattle (clipping not present).

Dickson informs Ewart that his gamekeeper has discovered omissions in Ewart's observations on animal hybridisation, and the emphasis on which parent is male and which female. He also says he is enclosing some notes about cross-breeding (not present).

Hagenbeck states that he will send Ewart a pair of black rats as soon as he is able to obtain them from the Zoological Gardens in Frankfurt. He is also sending him some photographs of the results of his male lion and tigress hybridisation experiments (not present).

Delamarre states that he had read about Ewart's researches in hybridisation in a volume of La Revue Scientifique and was enclosing a photograph of an animal which was currently living in the zoological gardens of the Bois de Boulogne (photograph not present).

Allen invites Ewart to prepare an article on the subject of animal breeding and hybridisation for inclusion in the Experiment Station Record.. He goes on to outline the possibilities of setting up a cooperative plan of animal experimentation between experiment stations across the country.

Cameron provides instances of hereditary physical characteristics passed on in the same family, and how this relates to hybridisation.

Shaw congratulates Ewart on the publication of the Penycuik Experiments. He discusses his own interest in the breeding of a pair of Burchell's zebras located at the Zoological Gardens in Bristol, and the markings of their offspring. He offers to send Ewart the skin of an 11 month-old foal for his inspection. He asks for advice on the hybridisation of zebras with ponies and donkeys in their care.

Jules de Guerne announces that the Société Nationale D'Acclimatation de France are bestowing upon Ewart the Grande Medaille d'Isidore Geoffroy Saint-Hilaire for his work on the hybridisation of zebras and horses.

Hope encloses a note (not present) from Mr Assheton Smith who she believes Ewart would be interested in contacting in relation to his hybridisation experiments. She mentions some friends in Norfolk who have a very old breed of cattle, and who are anxious to discover exactly from which breed of wild cattle they are likely to have descended.